Forschungsmodul: Proteomics VL+UE (WiSe 2014)
Welcome to the Wiki of the MSc lecture
Proteomics (VL: 19400901, UE: 19400902)
In this lecture we will cover the basics of HPLC/MS data generation as well as methods and algorithms for peptide and protein identificaiton and quantitative proteomics. In addition, we will cover statistical methods for assessing the significance of the results.
News
- The lab excursion will take place at Max Planck Institute for Moleculer Genetics (Ihnestr. 73, 14195). We will meet in front of the institute (new building, tower 4) at 1 p.m. on 04.11.2014. It will take at maximum 3 hours.
- 6.11.2014: There is an error in exercise sheet 2. Please look on the page of Lecture 2 for more info.
- Do not forget to consider watching the lectures at Eberhard-Karls-Universität Tübingen for another view/different explanations on the concepts of the lecture. Complete lecture given by Oliver Kohlbacher et al.
- The first review will take place tomorrow at 14:15 in Takustr. 9 room SR 053. Please be there at 14:00. You should bring a basic calculator but might also be good without one. The planned duration is 60 minutes. See you tomorrow.
- Results of the first review are now online on the bottom of the page!
- We added solutions to exercise 8
- Results of the second review are online. Students scoring in the range of 45-50% may contact the exercise instructors for a reexamination.
SWS: 2+1
Lecturer: Knut Reinert
Exercises: Julianus Pfeuffer, Xiao Liang
Language: English
Requirements for "Aktive Teilnahme"
Have over 50% of the points in reviews.
Times and Places
Schedule
Date |
Lecture |
Lecturer |
14.10.2014 |
Lecture 0: Admin/Intro |
Reinert |
21.10.2014 |
Lecture 1: Intro to Proteomics & Metabolomics |
Reinert |
28.10.2014 |
Lecture 2: Chromatography and mass spectrometry |
Reinert |
11.11.2014 |
Lecture 3: Basic statistics for computational MS |
Reinert |
18.11.2014 |
Lecture 4: Quant I: concepts, isobaric tags |
Reinert |
25.11.2014 |
Lecture 5: Quant II: LFQ, SILAC |
Reinert |
02.12.2014 |
Lecture 6: Quant III: MRM, SWATH |
Reinert |
09.12.2014 |
Lecture 7: Peptide ID I: DB search |
Reinert |
06.01.2015 |
Lecture 8: Peptide ID II: de novo ID I |
Reinert |
13.01.2015 |
Lecture 9: Protein inference |
Reinert |
20.01.2015 |
Lecture 10: Post-translational modifications |
Reinert |
27.01.2014 |
Lecture 11: Metabolite ID |
Reinert |
10.02.2015 |
Lecture 12: Metabolite quantification |
Reinert |
04.11.2014 |
Lab excursion (MPI) |
Pfeuffer/Liang |
14.10.-16.10. |
Basics |
18.11.-02.12. |
Quantitative Proteomics |
05.12.2014 |
Review 1 (during exercises) |
09.10.-13.01. |
Peptide and Protein ID |
27.01.-10.02. |
Metabolites |
03.02.2014 |
2nd review (during lecture time) |
Reviews
Results Review 1
MatrNr |
Pts |
4453598 |
91 |
4477260 |
94 |
4377500 |
58 |
4179384 |
45 |
4312427 |
26 |
4278301 |
44 |
Results Review 2
MatrNr |
Pts |
4453598 |
39 |
4477260 |
31 |
4377500 |
81 |
4179384 |
75 |
4312427 |
00 |
4278301 |
47 |