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Forschungsmodul: Proteomics VL+UE (WiSe 2014)

Welcome to the Wiki of the MSc lecture Proteomics (VL: 19400901, UE: 19400902)

In this lecture we will cover the basics of HPLC/MS data generation as well as methods and algorithms for peptide and protein identificaiton and quantitative proteomics. In addition, we will cover statistical methods for assessing the significance of the results.

News

  • The lab excursion will take place at Max Planck Institute for Moleculer Genetics (Ihnestr. 73, 14195). We will meet in front of the institute (new building, tower 4) at 1 p.m. on 04.11.2014. It will take at maximum 3 hours.
  • 6.11.2014: There is an error in exercise sheet 2. Please look on the page of Lecture 2 for more info.
  • Do not forget to consider watching the lectures at Eberhard-Karls-Universität Tübingen for another view/different explanations on the concepts of the lecture. Complete lecture given by Oliver Kohlbacher et al.
  • The first review will take place tomorrow at 14:15 in Takustr. 9 room SR 053. Please be there at 14:00. You should bring a basic calculator but might also be good without one. The planned duration is 60 minutes. See you tomorrow.
  • Results of the first review are now online on the bottom of the page!
  • We added solutions to exercise 8
  • Results of the second review are online. Students scoring in the range of 45-50% may contact the exercise instructors for a reexamination.

General Information

SWS: 2+1
Lecturer: Knut Reinert
Exercises: Julianus Pfeuffer, Xiao Liang
Language: English

Requirements for "Aktive Teilnahme"

Have over 50% of the points in reviews.

Times and Places

Event Day Time Address Room
Lecture Tue 14-16 Arnimallee 3 HS 001
Exercise Fri 14-16 Takustr. 9 SR 053

Schedule

Date Lecture Lecturer
14.10.-16.10. Basics
14.10.2014 Lecture 0: Admin/Intro Reinert
21.10.2014 Lecture 1: Intro to Proteomics & Metabolomics Reinert
28.10.2014 Lecture 2: Chromatography and mass spectrometry Reinert
04.11.2014 Lab excursion (MPI) Pfeuffer/Liang
11.11.2014 Lecture 3: Basic statistics for computational MS Reinert
18.11.-02.12. Quantitative Proteomics
18.11.2014 Lecture 4: Quant I: concepts, isobaric tags Reinert
25.11.2014 Lecture 5: Quant II: LFQ, SILAC Reinert
02.12.2014 Lecture 6: Quant III: MRM, SWATH Reinert
05.12.2014 Review 1 (during exercises)
09.10.-13.01. Peptide and Protein ID
09.12.2014 Lecture 7: Peptide ID I: DB search Reinert
06.01.2015 Lecture 8: Peptide ID II: de novo ID I Reinert
13.01.2015 Lecture 9: Protein inference Reinert
20.01.2015 Lecture 10: Post-translational modifications Reinert
27.01.-10.02. Metabolites
27.01.2014 Lecture 11: Metabolite ID Reinert
03.02.2014 2nd review (during lecture time)
10.02.2015 Lecture 12: Metabolite quantification Reinert

Reviews

Results Review 1

MatrNr Pts
4453598 91
4477260 94
4377500 58
4179384 45
4312427 26
4278301 44

Results Review 2

MatrNr Pts
4453598 39
4477260 31
4377500 81
4179384 75
4312427 00
4278301 47
Topic revision: r17 - 03 Feb 2015, jpfeuffer
 
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